This technology provides a simple and efficient strategy for targeted gene mutation and thereby will accelerating the pace of research of pathogenicity and development in this economically important plant pathogen. The U6-sgRNA system exhibited a significantly higher efficiency of gene mutation at both loci. However, neither system successfully generated mutations at the Sspks12 gene locus. This item: Neenah Creative Collection Paper, 8.5' x 11', 70 lb/189 GSM, Double Color Assortment, 25 Sheets (91208), 21.33 x 27.68 x 0.76 cm, Multicolor, Count 8.51 (0.34/Count) Only 1 left in stock - order soon. The RNP system, similar to the TrpC-sgRNA system we previously reported, creates mutations at the Ssoah1 gene locus with comparable efficiency. The previously characterized oxaloacetate acetylhydrolase ( Ssoah1) locus and a new locus encoding polyketide synthase12 ( Sspks12) were targeted in this study to create loss-of-function mutants. Medium paper weight offers a sturdy feel for long-lasting use. To further optimize the CRISPR-Cas9 system, a plasmid-free Cas9 protein/sgRNA ribonucleoprotein (RNP)-mediated system (the RNP system) and a plasmid-based RNA polymerase III promoter (U6)-driven sgRNA transcription system (the U6-sgRNA system) were established and evaluated. Neenah Paper Creative Collection Premium Cardstock, 65 lb, 8.5 x 11, Assorted Starter Pack, 72/Pack Design long-lasting projects with this acid-free premium cardstock that resists fading. However, relatively low efficiency targeted gene editing hampered the application of this method for functional genomic research in S. From colorful classroom projects to paper. Plus, it’s lignin and acid-free so that it won’t yellow over time. Each sheet features an on-trend vibrant color for everyday and special occasion projects, and is compatible with inkjet, and laser printers for striking and impressive prints. See Full Product Description See Product Specification. Neenah Creative Collection Specialty Cardstock Starter Kit, 12' x 12', 65 lb, 18-Color Assortment, 72 Sheets Sales Stats. This system (the TrpC-sgRNA system), based on an RNA polymerase II (RNA Pol II) promoter (TrpC) to drive sgRNA transcription in vivo, was successful in creating gene insertion mutants. Kick start your brightest ideas with the Neenah Creative Collection Specialty Cardstock Starter Kit. Neenah Paper Creative Collection Premium Cardstock 65 lb, 8.5 x 11, Assorted Starter Pack, 72/Pack 8.38/Pack. Neenah Creative Collection Specialty Cardstock Starter Kit, 12' x 12', 65 lb, 18-Color Assortment, 72 Sheets. We previously reported on a CRISPR-Cas9 genome editing system for the necrotrophic fungal plant pathogen Sclerotinia sclerotiorum. Neenah Creative Collection Specialty Cardstock Starter Kit, 12' x 12', 65 lb, 18-Color Assortment, 72 Sheets.
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